Y27632

Description Product Code Price Quantity Add to Cart
2mg (10mM in 624.4μl DMSO)
MD-0025
$146.00

Product Description

Y27632 is a cell-permeable small molecule Rho-associated kinase (ROCK) inhibitor [1]. Y27632 has been found to prevent apoptosis as well as enhance the survival and cloning efficiency of dissociated human embryonic stem (ES) cells without affecting their self-renewal properties or pluripotency [2]. This molecule has also been shown to enhance survival during the transplantation of ES cell-derived neural precursors [3]. Y27632 in combination with Pifithrin-µ significantly improves cell recovery after cryopreservation [4].

 
  Catalog No.:      MD-0025
  Quantity:      2mg (10mM in 624.4μl DMSO)
  Alternate Name:     (1R,4r)-4-((R)-1-aminoethyl)-N-(pyridine-4-yl)cyclohexanecarboxamide dihydrochloride
  CAS Number:    146986-50-7
  Chemical Formula:   C14H23Cl2N3O
  Molecular Weight:   320.3 g/mol
  Purity:   >Greater than 98% by HPLC analysis
  Structure:   Y-27632
  Applications:   MAINTENANCE AND SELF-RENEWAL
  • Enhances survival of human embryonic stem (ES) cells when they are dissociated to single cells by preventing dissociation-induced apoptosis (anoikis), thus increasing their cloning efficiency.
  • Improves embryoid body formation using forced-aggregation protocols.
  • Increases the survival of cryopreserved single human ES cells after thawing.
  • Blocks apoptosis of mouse ES-derived neural precursors after dissociation and transplantation.

  REPROGRAMMING

  • Direct lineage reprogramming of fibroblasts to mature neurons, in combination with CHIR99021, RepSox, Forskolin, SP600125, Gö6983 and Valproic Acid.

  DIFFERENTIATION

  • Improves survival of human ES cell monolayers at the initiation of differentiation protocols
  Product Use:   Y27632 is for research use only.  It is not approved for human or animal use, or for application in in vitro diagnostic procedures.
  Storage & stability:   Store aliquots at -20°C. Stock solutions are stable for 6 months when stored as directed.

 

References

[1] Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.

[2] Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.

[3] Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.

[4] Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.

[1] Ishizaki, T., Uehata, M., Tamechika, I., Keel, J., Nonomura, K., Maekawa, M., and Narumiya, S. (2000) Pharmacological properties of Y-27632, a specific inhibitor of rho-associated kinases. Mol Pharmacol 57: 976-983.

[2] Watanabe, K., Ueno, M., Kamiya, D., Nishiyama, A., Matsumura, M., Wataya, T., Takahashi, J.B., Nishikawa, S., Nishikawa, S., Muguruma, K., and Sasai, Y. (2007) A ROCK inhibitor permits survival of dissociated human embryonic stem cells.Nat Biotechnol 25: 681-686.

[3] Koyanagi, M., Takahashi, J., Arakawa, Y., Doi, D., Fukuda, H., Hayashi, H., Narumiya, S., and Hashimoto, N. (2008) Inhibition of the Rho/ROCK pathway reduces apoptosis during transplantation of embryonic stem cell-derived neural precursors.J. Neurosci Res. 86: 270-280.

[4] Xu, X., Cowley, S., Flaim, C.J., James, W., Seymour, L.W., Cui, Z. (2010) Enhancement of cell recovery for dissociated human embryonic stem cells after cryopreservation. Biotechnol Prog. 26: 781-788.