CD40L/CD154/TRAP, Human
| Description | Product Code | Price | Quantity | Add to Cart |
|---|---|---|---|---|
|
10ug
|
IX02022-10
|
$65.00
|
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|
50ug
|
IX02022-50
|
$159.00
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1mg
|
IX02022-1
|
$1,049.00
|
Overview
| Synonyms | CD40 Ligand, CD154, TRAP, TNFSF5, gp39 |
| Description | CD40 Ligand (CD40L/CD154/TRAP) is a membrane glycoprotein and differentiation antigen expressed on the surface of T-cells. The CD40 ligand stimulates B-cell proliferation and secretion of all immunoglobulin isotypes in the presence of cytokines. It also costimulates proliferation of activated T-cell and this is accompanied by the production of IFN-γ, TNF-α, and IL2. CD40 ligand has been shown to induce cytokine production and tumoricidal activity in peripheral blood monocytes. Recombinant Human CD40 Ligand (CD40L/CD154/TRAP) produced in E. coli is a non-glycosylated, polypeptide chain containing 149 amino acids and having a molecular mass of 16,308 Da. |
| Species | Human |
| Source | E. coli |
| Biological Activity | Recombinant human CD40 Ligand (CD40L/CD154/TRAP) is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of IL-12 & IL-8 induction by PMB (Peripheral Mononuclear) cells was found to be 5-10 ng/ml, corresponding to a Specific Activity of 1.0 x 105 IU/mg. |
Properties
| Measured Molecular Weight | 16,308 Da |
| Purity | Greater than 95.0% as determined by: 1. Analysis by RP-HPLC. 2. Anion-exchange FPLC. 3. Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Formulation | The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH8.0, 250mM NaCl buffer. |
| Reconstitution | It is recommended to reconstitute the lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Endotoxin Level | Less than 0.1 ng/µg (1 EU/µg) of recombinant human CD40 Ligand (CD40L) as determined by LAL test. |
| Sequence Analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Gln-Lys-Gly-Asp. |
| Storage | Lyophilized recombinant human CD40 Ligand (CD40L/CD154/TRAP) although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution CD40L should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Note | ; |
| Biological | |
|---|---|
| Source | E. coli |
| Species | Human |