EGF, Human

Description Product Code Price Quantity Add to Cart
10ug
IX00333-10
$25.00
50ug
IX00333-50
$75.00
1mg
IX00333-1
$140.00

Overview

Synonyms Human Epidermal Growth Factor
Description Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues.
Species Human
Source E. coli
Biological Activity The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of 5.0×106 IU/ mg.
Sequence
00001 MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG
00041 ERCQYRDLKW WELR

Properties

Measured Molecular Weight 6.2 kDa+/-10% determined by reduced SDS-PAGE.
Purity Greater than 95% as determined by Analysis by SDS-PAGE and SEC-HPLC.
Formulation Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200mM NaCl buffer.
Reconstitution It is recommended to reconstitute the lyophilized recombinant human Epidermal Growth Factor (EGF) in ddH2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin Level Less than 0.2 EU/μg of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test.
Sequence Analysis The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met- Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His-Asp-Gly-Tyr-Cys.
Storage Lyophilized recombinant Human Epidermal Growth Factor (rhEGF) remains stable up to 12months at lower than -70°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.
Biological
Source E. coli
Species Human