EGF, Human
| Description | Product Code | Price | Quantity | Add to Cart |
|---|---|---|---|---|
|
10ug
|
IX00333-10
|
$32.00 | ||
|
50ug
|
IX00333-50
|
$95.00 | ||
|
1mg
|
IX00333-1
|
$175.00 |
Overview
| Synonyms | Human Epidermal Growth Factor |
| Description | Epidermal Growth Factor (EGF) is a polypeptide growth factor which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant human Epidermal Growth Factor (EGF) is a 6,200 Da protein containing 53 amino acid residues. |
| Species | Human |
| Source | E. coli |
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of murine BALB/c 3T3 cells is less than 0.2 ng/ml, corresponding to a specific activity of 5.0×106 IU/ mg. |
| Sequence |
00001 MNSDSECPLS HDGYCLHDGV CMYIEALDKY ACNCVVGYIG
00041 ERCQYRDLKW WELR |
Properties
| Measured Molecular Weight | 6.2 kDa+/-10% determined by reduced SDS-PAGE. |
| Purity | Greater than 95% as determined by Analysis by SDS-PAGE and SEC-HPLC. |
| Formulation | Recombinant human Epidermal Growth Factor (EGF) was lyophilized after extensive dialysis against 10mM Phosphate buffer, pH 7.0, 200mM NaCl buffer. |
| Reconstitution | It is recommended to reconstitute the lyophilized recombinant human Epidermal Growth Factor (EGF) in ddH2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Endotoxin Level | Less than 0.2 EU/μg of recombinant human Epidermal Growth Factor (EGF) as determined by LAL test. |
| Sequence Analysis | The sequence of the first fifteen N-terminal amino acids was determined and was found to be Met- Asn-Ser-Asp-Ser-Glu-Cys-Pro-Leu-Ser-His-Asp-Gly-Tyr-Cys. |
| Storage | Lyophilized recombinant Human Epidermal Growth Factor (rhEGF) remains stable up to 12months at lower than -70°C from date of receipt. Upon reconstitution, rhEGF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C. |
| Biological | |
|---|---|
| Source | E. coli |
| Species | Human |
