Eotaxin-2/CCL24, Human

Description Product Code Price Quantity Add to Cart
20ug
IX02846-20
$159.00
1mg
IX02846-1
$2,100.00

Overview

Synonyms Eotaxin-2/CCL24, Human;
Description Eotaxin, also named MPIF-2 and Ckß6, is a novel CC chemokine recently identified. It is produced by activated monocytes and T lymphocytes. Eotaxin-2 selectively chemoattracts cells expressing CCR3 including eosinophils, basophils, Th2 T cells, mast cells, and certain subsets of dendritic cells. Additionally, Eotaxin-2 inhibits the proliferation of multipotential hematopoietic progenitor cells. The mature protein, which also includes a C-terminal truncation, contains 78 amino acid residues (92 a.a. residues for the mouse homolog, without C-terminal truncation).
Species Human
Source E. coli
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-100 ng/ml.
Sequence
VVIPSPCCMF FVSKRIPENR VVSYQLSSRS TCLKAGVIFT
TKKGQQFCGD PKQEWVQRYM KNLDAKQKKA SPRARAVA

Properties

Measured Molecular Weight Approximately 8.8 kDa, a single non-glycosylated polypeptide chain containing 78 amino acids.
Purity > 97 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rHuEotaxin-2/CCL24 as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;
Biological
Source E. coli
Species Human