FGF-basic (154aa), Human

Description Product Code Price Quantity Add to Cart
10ug
IX03116-10
$40.00
50ug
IX03116-50
$85.00
1mg
IX03116-1
$625.00
10 ug
IX03116-10
$625.00
1 mg
IX03116-1000
$1,225.00

Overview

Synonyms Fibroblast Growth Factor-basic, FGF-2, HBGF-2, Prostatropin
Description Fibroblast Growth Factor-basic (FGF-basic), also known as FGF-2, is a pleiotropic cytokine and one of the prototypic members of the heparin-binding FGF family. Like other FGF family members, bFGF has the β trefoil structure. In vivo, bFGF is produced by a variety of cells, including cardiomycotes, fibroblasts, and vascular cells. bFGF regulates a variety of processes including cell proliferation, differentiation, survival, adhesion, motility, apoptosis, limb formation and wound healing. bFGF can be tumorigenic due to its role in angiogenesis and blood vessel remodeling. The angiogenic effects of bFGF can produce beneficial cardioprotection during acute heart injury.
Recombinant human Fibroblast Growth Factor-basic (rhFGF-basic) produced in E. coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rhFGF-basic has a molecular mass of 17.1 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
Accession No P09038
Species Human
Source E. coli
Biological Activity ED50 < 0.25 ng/mL, measured by the cell proliferation assay using 3T3 cells, corresponding to a specific activity of > 4 × 106 units/mg.
Sequence
AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL
RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR
YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT
SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS

Properties

Measured Molecular Weight 17.1 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE analysis.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant human Fibroblast Growth Factor-basic (rhFGF-basic) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhFGF-basic remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only
Biological
Source E. coli
Species Human