GM-CSF, Human

Description Product Code Price Quantity Add to Cart
1mg
IX00349-1
$2,100.00

Overview

Synonyms rHuGM-CSF; GM-CSF
Description Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is produced by a number of different cell types, including activated T cells, B cells, macrophages, mast cells, endothelial cells, and fibroblasts, in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) is a growth factor for erythroid, megakaryocyte, and eosinophil progenitors. On mature hematopoietic, monocytes/macrophages and eosinophils.
Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can induce human endothelial cells to migrate and proliferate. Additionally, Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) can stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma, and adenocarcinoma cell lines.
Species Human
Source E. coli
Biological Activity The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.1 ng/ml, corresponding to a Specific Activity of 1.0×107 IU/mg.
Sequence
MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET
VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM
MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF
DCWEPVQE

Properties

Measured Molecular Weight 14,000 Da
Purity Greater than 98% as determined by
(a) Analysis by SEC-HPLC
(b) Analysis by reducing and non-reducing SDS-PAGE silver-stained gel
Formulation The protein was lyophilized after extensive dialysis against 20mM Phosphate buffer, pH7.0, 150mM NaCl, 5% Mannitol buffer.
Reconstitution It is recommended that the lyophilized Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) be reconstituted in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions.
Endotoxin Level Less than 0.03 ng/µg (0.3 IEU/μg) determined by LAL test
Sequence Analysis The sequence of the first fifteen N-terminal amino acids has been found to be (Met)-Ala-Pro-Ala-Arg-Ser-Pro-Ser-Pro-Ser-Thr-Gln-Pro-Trp-Glu-His.
UV The maximal absorption wave is 279+/-3 nm.
Storage Lyophilized Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF), although stable at room temperature for three weeks, should be stored desiccated at -18°C or below. Upon reconstitution, Recombinant Human Granulocyte Macrophage-Colony Stimulating Factor (GM-CSF) may be stored at 4°C for short periods (two to seven days). For long term storage, it is recommended that a carrier protein (0.1% HSA or BSA) be added. Avoid repeated freeze-thaw cycles.
Note ;
Biological
Source E. coli
Species Human