GM-CSF, Human (P. pastoris-expressed)
| Description | Product Code | Price | Quantity | Add to Cart |
|---|---|---|---|---|
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10ug
|
IX02190-10
|
$95.00
|
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|
50ug
|
IX02190-50
|
$325.00
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1mg
|
IX02190-1
|
$2,250.00
|
Overview
| Synonyms | rHu GM-CSF, Sargramostim, Recombinant Human GM-CSF, Sargramostim; |
| Description | Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) was initially characterized as a growth factor that can support the in vitro colony formation of granulocyte-macrophage progenitors. It is produced by a number of different cell types (including activated T cells, B cells, macrophages, mast cells, endothelial cells and fibroblasts) in response to cytokine of immune and inflammatory stimuli. Besides granulocyte-macrophage progenitors, GM-CSF is also a growth factor for erythroid, megakaryocyte and eosinophil progenitors. On mature hematopoietic, monocytes/ macrophages and eosinophils. GM-CSF has also been reported to have a functional role on non-hematopoitic cells. It can induce human endothelial cells to migrate and proliferate. Additionally, GM-CSF can also stimulate the proliferation of a number of tumor cell lines, including osteogenic sarcoma, carcinoma and adenocarcinoma cell lines. GenScript Human Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) (Sargramostim), is a single, glycosylated polypeptide chain containing 127 amino acids and having a molecular mass of about 24,000-35,000 Da, as shown in SDS-PAGE. It differs from natural human GM-CSF by a substitution of leucine at position 23 (R to L), and the carbohydrate moiety may be different from the native protein. |
| Species | Human |
| Source | P. pastoris |
| Sequence | The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. |
Properties
| Specificity | The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is less than 0.2 ng/ml, corresponding to a specific activity of 5.0×106 IU/ mg. |
| Measured Molecular Weight | 24,000-35,000 Da |
| Purity | The purity level of GenScript Recombinant Human GM-CSF is greater than 95.0%, as determined by the following methods: (a) RP-HPLC anaylsis (b) Reducing and non-reducing SDS-PAGE silver-stained gel analysis |
| Formulation | The protein was lyophilized after extensive dialysis against 10mM Tris-HCl, pH8.5, 4% Mannitol, 1% Sucrose buffer. |
| Reconstitution | It is recommended to reconstitute the lyophilized Sargramostim in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Endotoxin Level | Less than 0.1 ng/µg (1 EU/µg) of sargramostim |
| Storage | Although lyophilized GenScript Sargramostim can remain stable at room temperature for three weeks, it is best stored desiccated below -18°C. Upon reconstitution Sargramostim should be stored at 4°C between 2-7 days and for future use below -18°C. For long-term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles. |
| Biological | |
|---|---|
| Source | P. pastoris |
| Species | Human |