GRO-β/CXCL2, Human

Description Product Code Price Quantity Add to Cart
10ug
IX02818-10
$159.00
1mg
IX02818-1
$2,100.00

Overview

Synonyms GRO-beta / CXCL2, Human;
Description The three GRO cDNAs encode 107 amino acid precursor proteins from which the N-terminal 34 amino acid residues are cleaved to generate the mature GROs. There are no potential N-linked glycosylation sites in the amino acid sequences. GRO expression is inducible by serum or PDGF and/or by a variety of inflammatory mediators, such as IL-1 and TNF, in monocytes, fibroblasts, melanocytes and epithelial cells. In certain tumor cell lines, GRO is expressed constitutively.Similar to other alpha chemokines, the three GRO proteins are potent neutrophil attractants and activators. In addition, these chemokines are also active toward basophils. All three GROs can bind with high affinity to the IL-8 receptor type B.
Species Human
Source E. coli
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human CXCR2 transfected human 293 cells is in a concentration range of 10-100 ng/ml.
Sequence
APLATELRCQ CLQTLQGIHL KNIQSVKVKS PGPHCAQTEV
IATLKNGQKA CLNPASPMVK KIIEKMLKNG KSN

Properties

Measured Molecular Weight Approximately 7.9 kDa, a single non-glycosylated polypeptide chain containing 73 amino acids.
Purity > 97 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 50 mM NaCl.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rHuGRO-β/CXCL2 as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;
Biological
Source E. coli
Species Human