40HU-102-ISO
Starting at:
Starting at: $690.00
Starting at: $128.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| IX02985-1 | 1mg | Starting at: $2,328.00 | ||
| IX02985-10 | 10ug | Starting at: $128.00 | ||
| IX02985-50 | 50ug | Starting at: $466.00 |
Starting at: $690.00
Starting at: $1,414.00
Starting at: $690.00
Starting at: $1,414.00
Starting at: $1,414.00
Starting at: $1,414.00
Starting at: $690.00
Overview
| Synonyms | Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF) |
| Description | Interleukin-1 Beta (IL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine. Recombinant mouse Interleukin-1 Beta(rmIL-1β) produced in E. coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rmIL-1β is obtained by proprietary chromatographic techniques, with an apparent molecular weight of 17.5kDa analyzed by reducing SDS-PAGE. |
| Species | Mouse |
| Source | E. coli |
| Biological Activity | ED50< 10 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, corresponding to a specific activity of 1.0×108 IU/mg. |
| Sequence |
MVPIRQLHYR LRDEQQKSLV LSDPYELKAL HLNGQNINQQ
VIFSMSFVQG EPSNDKIPVA LGLKGKNLYL SCVMKDGTPT LQLESVDPKQ YPKKKMEKRF VFNKIEVKSK VEFESAEFPN WYISTSQAEH KPVFLGNNSG QDIIDFTMES VSS |
Properties
| Measured Molecular Weight | 17.5kDa, observed by reducing SDS-PAGE. |
| Purity | > 95% by SDS-PAGE and HPLC analyses. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Endotoxin Level | <0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant mouseInterleukin-1 Beta(rmIL-1β) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmIL-1β should be stable up to 2 weeks at 4°C or up to 3 months at -20°C. |
| Note | For research use only |
Starting at: $690.00
Starting at: $1,414.00
Starting at: $690.00
Starting at: $1,414.00
FREQUENTLY BOUGHT TOGETHER - RECOMMENDED FOR YOU!
Starting at: $690.00
Starting at: $1,414.00
Starting at: $690.00
Starting at: $1,414.00
Starting at: $1,414.00
Starting at: $1,414.00