IL-15, Human
| Description | Product Code | Price | Quantity | Add to Cart |
|---|---|---|---|---|
|
10ug
|
IX00377-10
|
$199.00 | ||
|
50ug
|
IX00377-50
|
$699.00 | ||
|
1mg
|
IX00377-1
|
$3,369.00 |
Overview
| Synonyms | IL-15, MGC9721 |
| Description | Interleukin 15 (IL-15) is a cytokine that possesses a variety of biological functions. Interleukin 15 (IL-15) stimulates the proliferation of T-lymphocytes, which requires interaction of IL-15 with components of IL-2R, including IL-2R and probably IL-2R gamma, but not IL-2R. Recombinant human Interleukin 15 (IL-15) produced in E. coli is a single, non-glycosylated polypeptide chain containing 114 amino acids and having a molecular mass of 12.5 kD. |
| Species | Human |
| Source | E. coli |
| Biological Activity | The ED50 as determined by the dose-dependant stimulation of the proliferation of CTLL-2 was found to be < 0.5 ng/ml, corresponding to a specific activity of > 2x106 IU/mg. |
| Sequence | NWV NVISDLKKIE DLIQSMHIDA TLYTESDVHP SCKVTAMKCF LLELQVISLE SGDASIHDTV ENLIILANNS LSSNGNVTES GCKECEELEE KNIKEFLQSF VHIVQMFINT S |
Properties
| Measured Molecular Weight | 12.5 kD |
| Purity | > 95% by SDS-PAGE and HPLC analyses. |
| Dimers | Less than 1% as determined by silver-stained SDS-PAGE gel analysis |
| Formulation | Recombinant human Interleukin-15 (IL-15) was lyophilized after extensive dialysis against 20mM PB, pH7.5, 150mM NaCl buffer. |
| Reconstitution | It is recommended to reconstitute the lyophilized Recombinant Human Interleukin 15 (IL-15) in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Quantitation | Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.55 as the extinction coefficient for a 0.1% (1 mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IL-15 as a Reference Standard. |
| Endotoxin Level | Less than 0.1 ng/µg (1 EU/µg) of recombinant human Interleukin-15 (IL-15) as determined by LAL test. |
| Sequence Analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Asn-Trp-Val-Asn-Val. |
| QC | Less than 1% as determined by silver-stained SDS-PAGE gel analysis. |
| Storage | Lyophilized recombinant human Interleukin 15 (IL-15) remains stable at room temperature for three weeks, but it is best stored desiccated below -18°C. Upon reconstitution recombinant human Interleukin 15 (IL-15) should be stored at 4°C for up to seven days. For long term storage it is recommended that a carrier protein (0.1% HSA or BSA ) be added. Avoid repeated freeze-thaw cycles. |
| Note | For in vitro use only |
| Biological | |
|---|---|
| Source | E. coli |
| Species | Human |
