IL-1RA, Human
| Description | Product Code | Price | Quantity | Add to Cart |
|---|---|---|---|---|
|
10ug
|
IX00367-10
|
$107.00 | ||
|
50ug
|
IX00367-50
|
$337.00 | ||
|
1mg
|
IX00367-1
|
$1,435.00 |
Overview
| Synonyms | rHuIL-1ra; IL1 Receptor Antagonist; Interleukin-1 Receptor Antagonist; Interleukin1 Receptor Antagonist |
| Description | Interleukin-1 receptor antagonist (IL-1ra) is a member of the IL-1 family. Endogenous IL-1ra is produced in numerous animal disease models as well as in human autoimmune and chronic inflammatory diseases. It binds to IL-1 receptors in competition with IL-1, but does not elicit intracellular response from this binding. Its role in counteracting the proinflammatory effects of IL-1 is being studied by numerous research groups. IL-4 and IL-13 have been shown to amplify the stimulatory effect of IL1-beta on the production of soluble and intracellular forms of IL1-ra. The regulated expression of IL1ra in various cell types has been shown to be influenced by cytokines. In synovial fibroblasts the synthesis of IL-1ra is markedly enhanced by IL-1 , TNF-alpha , or PDGF. Recombinant Human IL-1RN produced in E. coli is a non-glycosylated, N-terminal methionyl form of the human naturally-occurring polypeptide chain containing 153 amino acids and having a molecular mass of 17,258 Da. |
| Species | Human |
| Source | E. coli |
| Biological Activity | The ED50 as determined by the dose-dependant inhibition of IL-1 stimulation of D10S cells was found to be 0.5 ng/ml, corresponding to a Specific Activity of 2.0×106 IU/mg. |
Properties
| Measured Molecular Weight | 17,258 Da |
| Purity | Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Anion-exchange FPLC. (c) Analysis by reducing and non-reducing SDS-PAGE Silver Stained gel. |
| Formulation | The protein was lyophilized after extensive dialysis against 50mM Tris-HCl, pH7.5, 200mM NaCl buffer. |
| Reconstitution | It is recommended to reconstitute the lyophilized rHuIL-1ra in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
| Quantitation | |
| Endotoxin Level | Less than 0.1 ng/µg (IEU/µg) of IL-1 ra. |
| Sequence Analysis | The sequence of the first five N-terminal amino acids was determined and was found to be Met-Arg-Pro-Ser-Gly. |
| Storage | Lyophilized rHuIL-1 ra although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution rHuIL-1ra should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid repeated freeze-thaw cycles. |
| Note | ; |
| Biological | |
|---|---|
| Source | E. coli |
| Species | Human |
