40HU-102-ISO
Starting at:
Starting at: $690.00
Starting at: $88.00
| SKU | Package Size | Price | Quantity | Add to Cart |
|---|---|---|---|---|
| IX02925-1 | 1mg | Starting at: $1,596.00 | ||
| IX02925-10 | 10ug | Starting at: $88.00 | ||
| IX02925-50 | 50ug | Starting at: $212.00 |
Starting at: $690.00
Starting at: $1,414.00
Starting at: $690.00
Starting at: $1,414.00
Starting at: $1,414.00
Starting at: $1,414.00
Starting at: $690.00
Overview
| Synonyms | IL-4, B cell growth factor-1 (BCGF-1), B cell stimulatory factor-1 (BSF-1), B cell differentiation factor (BCDF), T cell growth factor-2 (TCGF-2), Mast-cell growth factor-2 (MCGF-2) |
| Description | Interleukin-4 (IL-4) is a pleiotropic cytokine regulates diverse T and B cell responses including cell proliferation, survival, and gene expression. It has important effects on the growth and differentiation of different immunologically competent cells. Interleukin-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of native CD4+ T cells (Th0 cells) into helper Th2 cells, and regulates the immunoglobulin class switching to the IgG1 and IgE isotypes. IL-4 has numerous important biological functions including stimulating B-cells activation, T-cell proliferation and CD4+ T-cells differentiation to Th2 cells. It is a key regulator in hormone control and adaptive immunity[2]. IL-4 also plays a major role in inflammation response and wound repair via activation of macrophage into M2 cells. IL-4 is stabilized by three disulphide bonds forming a compact globular protein structure. Four alpha-helix bundle with left-handed twist is dominated half of the protein structure with 2 overhand connections and fall into a 2-stranded anti-parallel beta sheet.
Recombinant human IL-4 (rhIL-4) is a 129 amino acid protein expressed in mammalian CHO system. The approximate molecular weight is 15 kDa analyzed by non-reducing SDS-PAGE. |
| Species | Human |
| Source | CHO |
| Biological Activity | ED50< 0.25 ng/ml, measured in a cell proliferation assay using TF-1 human erythroleukemic cells, corresponding to a specific activity of >4 x 106 units/mg |
| Sequence |
HKCDITLQEI IKTLNSLTEQ KTLCTELTVT DIFAASKNTT
EKETFCRAAT VLRQFYSHHE KDTRCLGATA QQFHRHKQLI RFLKRLDRNL WGLAGLNSCP VKEANQSTLE NFLERLKTIM REKYSKCSS |
Properties
| Measured Molecular Weight | 15 kDa, observed by non-reducing SDS-PAGE. |
| Purity | > 95% as analyzed by SDS-PAGE and HPLC. |
| Formulation | Lyophilized after extensive dialysis against PBS. |
| Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
| Endotoxin Level | <0.2 EU/μg, determined by LAL method. |
| Storage | Lyophilized recombinant human Interleukin 4 (IL-4) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-4 should be stable up to 1 week at 4°C or up to 2 months at -20°C。 |
Starting at: $690.00
Starting at: $1,414.00
Starting at: $690.00
Starting at: $1,414.00
FREQUENTLY BOUGHT TOGETHER - RECOMMENDED FOR YOU!
Starting at: $690.00
Starting at: $1,414.00
Starting at: $690.00
Starting at: $1,414.00
Starting at: $1,414.00
Starting at: $1,414.00