Description
Overview
Synonyms | CSF1, CSF-1, MCSF |
Description | Human Macrophage-Colony Stimulating Factor (M-CSF), also known as Colony Stimulating Factor-1 (CSF-1), can stimulate the survival, proliferation and differentiation of mononuclear phagocytes, in addition to the spreading and motility of macrophages. M-CSF is mainly produced by monocytes, macrophages, fibroblasts, and endothelial cells. M-CSF interaction with its receptor, c-fms, has been implicated in the growth, invasion, and metastasis of of several diseases, including breast and endometrial cancers. |
Species | Human |
Source | CHO |
Biological Activity | ED50< 5 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 2×105 units/mg. |
Sequence |
EEVSEYCSHM IGSGHLQSLQ RLIDSQMETS CQITFEFVDQ
EQLKDPVCYL KKAFLLVQDI MEDTMRFRDN TPNAIAIVQL QELSLRLKSC FTKDYEEHDK ACVRTFYETP LQLLEKVKNV FNETKNLLDK DWNIFSKNCN NSFAECSSQG HERQSEGS |
Properties
Measured Molecular Weight | 32-40 kDa, observed by non-reducing SDS-PAGE. |
Purity | > 95% as analyzed by SDS-PAGE and HPLC. |
Formulation | Lyophilized after extensive dialysis against PBS. |
Reconstitution | Reconstituted in ddH2O or PBS at 100 μg/ml. |
Endotoxin Level | <0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant human Macrophage-Colony Stimulating Factor (rhM-CSF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhM-CSF should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
Note | For research use only |