Description
Overview
Synonyms | Macrophage Colony Stimulating Factor, CSF-1, MGI-IM, M-CSF |
Description | Macrophage Colony Stimulating Factor (M-CSF) ,also known as CSF1,is a potent hematopoietic factor produced by a variety of cells including lymphocytes, monocytes, fibroblasts, endothelial cells, myoblasts and osteoblasts. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. It is a key regulator of cellular proliferation, differentiation, and survival of blood monocytes, tissue macrophages and their progenitor cells.M-CSF affects macrophages and monocytes in several ways, including stimulating increased phagocytic and chemotactic activity, and increased tumour cell cytotoxicity.M-CSF is clinically used in the treatment of infection, malignancies and atherosclerosis. It facilitates hematopoietic recovery after bone marrow transplantation. Recombinant mouse Macrophage Colony Stimulating Factor (M-CSF) produced in E. coli is a disulfide-linked homodimer containing two non-glycosylated polypeptide chains of 156 amino acids each. A fully biologically active molecule, rmM-CSF has a molecular mass of 30 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques. |
Accession No | P07141 |
Species | Mouse |
Source | E. coli |
Biological Activity | ED50< 3 ng/ml, measured in a cell proliferation assay using Murine M-NFS-60 cells, corresponding to a specific activity of > 3.3×105 units/mg. |
Sequence |
MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD
QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP |
Properties
Measured Molecular Weight | 30 kDa, observed by reducing SDS-PAGE. |
Purity | > 95% as analyzed by SDS-PAGE. |
Formulation | Lyophilized after extensive dialysis against 50mMTris,150mMNaCl, pH 8.0. |
Reconstitution | Reconstituted in ddH2O at 100 μg/ml. |
Endotoxin Level | < 0.2 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant Mouse Macrophage Colony Stimulating Factor(M-CSF) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Mouse M-CSF should be stable up to 1 week at 4°C or up to 2 months at -20°C. |
Note | For research use only |