PreScission Protease

Description Product Code Price Quantity Add to Cart
5000IU
IX02799-5000
$0.00
100IU
IX02799-100
$75.00
250IU
IX02799-250
$159.00

Overview

Synonyms PreScission Protease(PSP);
Description PreScission Protease is a fusion protein of glutathione S-transferase (GST) and human rhinovirus (HRV) type 14 3C protease . The protease specifically recognizes a subset of sequences which include the core amino acid sequence Leu-Phe-Gln/Gly-Pro cleaving between the Gln and Gly residues . Substrate recognition and cleavage are likely to be dependent not only upon primary structural signals, but also upon the secondary and tertiary structures of the fusion protein as well.
Source E. coli
Biological Activity Unit Definition:
One unit is defined as the amount of enzyme needed to cleave 100 μg of fusion protein in 16 hours to 90% completion at 5°C in a buffer containing 50 mM Tris-HCl, pH 7.0, 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Cleavage Buffer:
50 mM Tris-HCl, pH 7.0 (at 25 °C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5 °C prior to use.

Properties

Reaction Buffer Cleavage Buffer:
50mM Tris-HCl, pH7.0 (at 25°C), 150 mM NaCl, 1 mM EDTA, 1 mM dithiothreitol. Chill to 5°C prior to use.
Physical Appearance Sterile colorless liquid.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE
Storage Should be stored in small aliquots at -20°C to -80°C for long term.
Note This material is offered for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Biological
Source E. coli