Protein G

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Synonyms Protein G; G Protein
Description Protein G, a cell surface protein of group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A from Staphylococcus aureus. This product contains three IgG binding domains, but its albumin-binding region has been removed. Additionally, His-tag and cysteine have been added to N- and C-termini, respectively, to facilitate purification and conjugation. This does not affect the protein's ability to bind to IgG.
Source E. coli
Biological Activity The theoretical binding capacity is 3 mol IgG/mol protein G. Protein G is assayed using ELISA. The optimal concentration for coating plate is 5 ug/ml.


Measured Molecular Weight 29,000-35,000 Da was determined by SDS-PAGE while relative molecular mass calculated from amino acid sequence is ≈24,000 Da.
Purity This product is chromatographically purified and shows predominantly a single band by SDS-PAGE.
Focusing: pI of 4.69
Concentration 5 mg/ml before lyophilization
Storage This product is lyophilized in 1X PBS. It will remain stable at 4°C for several weeks. For longer periods, store it at -20°C.
Note ;
Source E. coli