SDF-1β/CXCL12, Rat

Description Product Code Price Quantity Add to Cart
10ug
IX02860-10
$159.00
1mg
IX02860-1
$2,100.00

Overview

Synonyms SDF-1 beta/CXCL12, Rat;
Description SDF-1ª and ß are stromal derived CXC chemokines, and signal through the CXCR4 receptor. SDF-1ª and ß chemoattract B and T cells, and have been shown to induce migration of CD34+ stem cells. Additionally, the SDF-1 proteins exert HIV suppressive activity in cells expressing the CXCR4 receptor.
Species Rat
Source E. coli
Biological Activity Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 100-200 ng/ml.
Sequence
KPVSLSYRCP CRFFESHVAR ANVKHLKILN TPNCALQIVA
RLKSNNRQVC IDPKLKWIQE YLDKALNKRL KM

Properties

Measured Molecular Weight Approximately 8.4 kDa, a single non-glycosylated polypeptide chain containing 72 amino acids.
Purity > 97 % by SDS-PAGE and HPLC analyses.
Formulation Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Endotoxin Level Less than 1 EU/μg of rRtSDF-1β/CXCL12β as determined by LAL method.
Physical Appearance Sterile Filtered White lyophilized (freeze-dried) powder.
Usage This material is for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Storage This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Note ;
Biological
Source E. coli
Species Rat