TFF3, Human
Description | Product Code | Price | Quantity | Add to Cart |
---|---|---|---|---|
20ug
|
IX02904-20
|
$159.00
|
||
1mg
|
IX02904-1
|
$2,100.00
|
Overview
Synonyms | Trefoil Factor 3 Human; TFF3 Human |
Description | The Trefoil Factor peptides (TFF1, TFF2 and TFF3) are secreted in the gastrointestinal tract, and appear to play an important role in intestinal mucosal defense and repair. TFF-3 is expressed by goblet cells and in the uterus, and has also been shown to express in certain cancers, including colorectal, hepatocellular, and in biliary tumors. TFF3 may be useful as a molecular marker for certain types of cancer, but its role, if any, in tumorigenesis is unknown. TFF3 also promotes airway epithelial cell migration and differentiation. |
Species | Human |
Source | E. coli |
Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10 μg/ml, corresponding to a specific activity of > 100 IU/mg. |
Sequence | EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK PLQEAECTF |
Properties
Measured Molecular Weight | Approximately 6.6 kDa, a single non-glycosylated polypeptide chain containing 59 amino acids, which includes a 40-amino acid trefoil motif containing three conserved intramolecular disulfide bonds. |
Purity | > 95 % by SDS-PAGE and HPLC analyses. |
Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
Endotoxin Level | Less than 1 EU/μg of rHuTFF3 as determined by LAL method. |
Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
Note | ; |
Biological | |
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Source | E. coli |
Species | Human |