TNF-α, Human

Description Product Code Price Quantity Add to Cart
10ug
IX01001-10
$49.00
50ug
IX01001-50
$95.00
1mg
IX01001-1
$625.00

Overview

Synonyms TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2
Description Tumor Necrosis Factor-alpha (TNF-a) is a homotrimer with a subunit molecular mass of 17.3 kDa. Tumor Necrosis Factor-alpha(TNF-a) plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, and autoimmune diseases; and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF has been found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn's disease, and rheumatoid arthritis as well as graft-versus-host disease.
Recombinant Human Tumor Necrosis Factor-alpha (TNF-α) produced in E.coli is a single non-glycosylated polypeptide chain containing 157 amino acids. A fully biologically active molecule, rhTNF-α has a molecular mass of 17.3 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Species Human
Source E. coli
Biological Activity ED50 < 30 pg/ml, measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D, corresponding to a specific activity of > 3.3 x 107 units/mg.
Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG
VELRDNQLVV PSEGLYLIYS QVLFKGQGCP STHVLLTHTI
SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL
GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Properties

Measured Molecular Weight 17.3 kDa, observed by reducing SDS-PAGE.
Purity > 98% as analyzed by SDS-PAGE and HPLC.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level Less than 0.2 EU/μg determined by LAL test.
Storage Lyophilized recombinant Human Tumor Necrosis Factor-alpha (TNF-α), remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, human TNF-α should be stable up to 1 week at 4°C or up to 3 months at -20°C.
Note For research use only
Biological
Source E. coli
Species Human