TNF-α, Mouse (P. pastoris-expressed)
|Synonyms||TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, Cachectin, DIF, TNFA, TNFSF2|
|Description||Sharing 79% sequence identity with human Tumor Necrosis Factor-alpha (hTNF-α), mouse Tumor Necrosis Factor-alpha (mTNF-α) is a cytokine mainly expressed by immune cells . A type II transmembrane protein, TNF-α is further proteolytically processed to a soluble form . The trimeric active TNF-α then exerts its diverse biological properties including gapoptosis, inflammation, autoimmunity and cell proliferation by binding to TNF Receptor 1 and 2 .
Recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) produced in P.pastoris is a fully biologically active polypeptide chain of 152 amino acids with a molecular mass of 17 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques.
|Biological Activity||ED50<0.01ng/ml, measured by cytotoxicity assay using L929 cells, corresponding to a specific activity of >1 x 108units/mg.|
SQNSSDKPVA HVVANHQVEE QLEWLSQRAN ALLANGMDLK
DNQLVVPADG LYLVYSQVLF KGQGCPDYVL LTHTVSRFAI
SYQEKVNLLS AVKSPCPKDT PEGAELKPWY EPIYLGGVFQ
LEKGDQLSAE VNLPKYLDFA ESGQVYFGVI AL
|Measured Molecular Weight||17kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||<1 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant mouse Tumor Necrosis Factor-alpha (rmTNF-α) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rmTNF-α should be stable up to 1 week at 4°C or up to 2 months at -20°C.|