VEGF164, Mouse (P. pastoris-expressed)

Description Product Code Price Quantity Add to Cart
20ug
IX02690-20
$95.00
100ug
IX02690-100
$349.00
1mg
IX02690-1
$1,500.00

Overview

Synonyms VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen.
Description Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product. Recombinant mouse Vascular Endothelial Growth Factor A164 (rmVEGF-A164) produced in Pichia pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rmVEGF-A164 has a molecular mass of 39kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques
Species Mouse
Source P. pastoris
Biological Activity ED50 <6.0 ng/ml, measured by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of > 1.7x 105 units/mg.
Sequence
MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP
DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI
MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC
SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC
DKPRR

Properties

Measured Molecular Weight 39kDa, observed by non-reducing SDS-PAGE.
Purity > 97% as analyzed by reducing SDS-PAGE
Formulation Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0.
Reconstitution Reconstituted in ddH2O at 100 μg/ml.
Endotoxin Level <1.0 EU/μg, determined by LAL method.
Storage Lyophilized recombinant mouse Vascular Endothelial Growth Factor A164 (rmVEGF-A164)remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rmVEGF-A164 should be stable up to 4 week at 4°C or up to 6 months at -20°C.
Biological
Source P. pastoris
Species Mouse