VEGF164, Rat (P. pastoris-expressed)
|Synonyms||Vascular Endothelial Growth Factor 164 (VEGF-164), VEGF, Vascular Permeability Factor (VPF)|
|Description||Vascular Endothelial Growth Factor A164 (VEGF-A164), a member of the cysteine knot growth factor, is one of major isoforms of VEGF-As. VEGF-As are endothelial cell-specific mitogens with angiogenic and vascular permeability-inducing properties. During maturation, rat VEGF-A is alternatively spliced to generate rVEGF-A120, rVEGF-A164 and rVEGF-A188 which correspond to hVEGF-A121, hVEGF-A165 and hVEGF-A189 in human, respectively (the numbers designate the amino acid residues). The active form of rVEGF-A164 is either a homodimeric or heterodimeric polypeptides which bind to the transmembrane tyrosine kinases receptors FLT1, FLK1 or KDR or to the non-tyrosine kinase neuropilin receptors NRP1/2.
Recombinant rat Vascular Endothelial Growth Factor A164 (rrVEGF-A164) produced in P. pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rrVEGF-A164 has a molecular mass of 38 kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques.
|Biological Activity||ED50<4 ng/ml, measured by cell proliferation assay of HUVEC.|
MAPTTEGEQK AHEVVKFMDV YQRSYCRPIE TLVDIFQEYP
DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNVTMQI
MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPENHCEPC
SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC
|Measured Molecular Weight||38 kDa, observed by non-reducing SDS-PAGE.|
|Purity||> 95% as analyzed by reducing SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||<1 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant rat Vascular Endothelial Growth Factor A164(rrVEGF-A164) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrVEGF-A164 should be stable up to 1 week at 4°C or up to 2 months at -20°C.|