Description
Overview
Synonyms | VPF, Folliculostellate cell-derived growth factor, Glioma-derived endothelial cell mitogen |
Description | Vascular Endothelial Growth Factor (VEGF) is a potent growth and angiogenic cytokine. It stimulates proliferation and survival of endothelial cells, and promotes angiogenesis and vascular permeability. Expressed in vascularized tissues, Vascular Endothelial Growth Factor (VEGF) plays a prominent role in normal and pathological angiogenesis. Substantial evidence implicates Vascular Endothelial Growth Factor (VEGF) in the induction of tumor metastasis and intra-ocular neovascular syndromes. Vascular Endothelial Growth Factor (VEGF) signals through the three receptors; fms-like tyrosine kinase (flt-1), KDR gene product (the murine homolog of KDR is the flk-1 gene product) and the flt4 gene product.Recombinant human Vascular Endothelial Growth Factor A165 (rhVEGF-A165) produced in Pichia pastoris is a disulfide-linked homodimer containing two polypeptide chains of 165 amino acids each. A fully biologically active molecule, rhVEGF-A165 has a molecular mass of 38.2kDa analyzed by non-reducing SDS-PAGE and is obtained by chromatographic techniques. |
Species | Human |
Source | P. pastoris |
Biological Activity | ED50 of 1-5ng/ml, measured by the dose-dependent stimulation of the proliferation of HUVEC cells, corresponding to a specific activity of 2x 105-1x 106 units/mg. |
Sequence |
APMAEGGGQN HHEVVKFMDV YQRSYCHPIE TLVDIFQEYP
DEIEYIFKPS CVPLMRCGGC CNDEGLECVP TEESNITMQI MRIKPHQGQH IGEMSFLQHN KCECRPKKDR ARQENPCGPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR |
Properties
Measured Molecular Weight | 38.2kDa, observed by non-reducing SDS-PAGE |
Purity | > 95% as analyzed by reducing SDS-PAGE. |
Formulation | Lyophilized after extensive dialysis against 25 mM HEPES and 150 mM NaCl, pH 7.0. |
Reconstitution | Reconstituted in ddH2O at 100 μg/ml. |
Endotoxin Level | <0.5 EU/μg, determined by LAL method. |
Storage | Lyophilized recombinant human Vascular Endothelial Growth Factor A165 (rhVEGF-A165) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhVEGF-A165 should be stable up to 4 week at 4°C or up to 6 months at -20°C. |