Human Bronchial Epithelial Cells (HBEpC)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 0.5 million cells/vial
10HU-093
$689.00

Product Description

The respiratory epithelium is composed of a mixed population of ciliated, nonciliated, and mucous-secreting cells from proximal to distal airways. The individual characteristics of these cells create not only an effective physical barrier against various noxious substances, but also a highly sophisticated host defense system by producing and releasing a large number of chemical mediators and cytokines [1]. The bronchial epithelium consists of the surface epithelial cells and mucus glands. The surface epithelial cells are made up of three principle cell types: basal, goblet, and ciliated cells, of which the latter two form a suprabasal columnar structure and are necessary for mucociliary clearance. Studies using human primary bronchial epithelial cells (HBEpC) have demonstrated that IL-4 and IL-13 stimulation can modify cellular proliferation, ciliary beating, and mucous production [2]. HBEpC proliferation is also regulated in part by EGF receptor signaling [3]. HBEpC provide an excellent model system to study all aspects of epithelial function and disease, particularly those related to airway viral infections, as well as tissue repair mechanisms, signaling changes and potential treatments relevant to lung injuries, mechanical and oxidative stress, inflammation, pulmonary diseases and smoking. 

Figure 1. Human Bronchial Epithelial Cells (HBEpC) (phase contrast).

iXCells Biotechnologies provides high quality HBEpC, which are isolated from human bronchi and cryopreserved at P1, with >0.5 million cells in each vial. HBEpC express cytokeratin-18, -19, and vimentin. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand in Epithelial Cell Growth Medium (Cat# MD-0041) under the condition suggested by iXCells Biotechnologies. 

Product Details

  Tissue

  Human bronchia

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Epithelial Cell Growth Medium (Cat# MD-0041)

References

[1]. Velden, V. H., and H. F. Versnel. 1998. Bronchial epithelium: morphology, function and pathophysiology in asthma. Eur. Cytokine Netw. 9:585-597. 
[2]. Kikuchi, T., Shively, J.D., Foley, J.S., Drazen, J.M., Tschumperlin, D.J. (2004) Differentiation-dependent responsiveness of bronchial epithelial cells to IL-4/13 stimulation. Am J Physiol Lung Cell Mol Physiol. 287:L119-26. 
[3]. Kim S, Schein AJ, Nadel JA.(2005) E-cadherin promotes EGFR-mediated cell differentiation and MUC5AC mucin expression in cultured human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 289:L1049-60.

 Download Datasheet

[1]. Velden, V. H., and H. F. Versnel. 1998. Bronchial epithelium: morphology, function and pathophysiology in asthma. Eur. Cytokine Netw. 9:585-597. 
[2]. Kikuchi, T., Shively, J.D., Foley, J.S., Drazen, J.M., Tschumperlin, D.J. (2004) Differentiation-dependent responsiveness of bronchial epithelial cells to IL-4/13 stimulation. Am J Physiol Lung Cell Mol Physiol. 287:L119-26. 
[3]. Kim S, Schein AJ, Nadel JA.(2005) E-cadherin promotes EGFR-mediated cell differentiation and MUC5AC mucin expression in cultured human airway epithelial cells. Am J Physiol Lung Cell Mol Physiol. 289:L1049-60.

Biological
Cell System Pulmonary Cell System
Cell Type Epithelial Cells
Species Human (Normal)