Human Colonic Epithelial Cells (HCoEpC)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 0.5 million cells/vial
10HU-096
$649.00

Product Description

The colorectum is a major organ in both malignant and nonmalignant diseases. Cells that line the colonic mucosal surface form a major mechanical barrier that separates the host’s internal milieu from the external environment. In addition to the well-established role of epithelial cells in ion transport, these cells appear to function as an integral component of the mucosal immune system. Human colonic epithelial cells (HCoEpC) can process and present antigens to T cells in vitro, and can be stimulated to express HLA class II and intercellular adhesion molecules in vivo [1]. They also respond to a broad array of cytokines with altered gene expression and growth characteristics [2]. In addition, normal colonic epithelial cells were found to express the α3, α5, α6, β1 and β4 integrin [3].

iXCells Biotechnologies provides high quality HCoEpC, which are isolated from human colonic tissue and cryopreserved at P1, with >0.5 million cells in each vial. HCoEpC express cytokeratin-18 and -19. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand for 10 population doublings in Epithelial Cell Growth Medium (Cat# MD-0041) under the condition suggested by iXCells Biotechnologies. 

Product Details

  Tissue

  Human colonic tissu

  Package Size

  0.5 million cells/vial  

  Passage Number

  P1

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Epithelial Cell Growth Medium (Cat# MD-0041)

References

[1] Mayer, L., Eisenhardt, D., Salomon, P., Bauer, W., Plous, R. and Piccinini, L. (1991) Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease. Gastroenterology. 100:3-12.
[2] Eckmann, L., Jung H.-C., Schuerer-Maly, C.-C., Panja, A., Morzycka-Wroblewska, E., and Kagnoff, M. F. (1993) Differential cytokine expression by human intestinal epithelial cell lines: regulated expression of interleukin-8. Gastroenterology. 105:1689-1697.
[3] Stallmach, A., v Lampe, B., Matthes, H., Bornhoft, B., and Riecken, E. O. (1992) Diminished expression of integrin adhesion molecules on human colonic epithelial cells during the benign to malign tumor. Gut 33:342-346.

[1] Mayer, L., Eisenhardt, D., Salomon, P., Bauer, W., Plous, R. and Piccinini, L. (1991) Expression of class II molecules on intestinal epithelial cells in humans. Differences between normal and inflammatory bowel disease. Gastroenterology. 100:3-12.
[2] Eckmann, L., Jung H.-C., Schuerer-Maly, C.-C., Panja, A., Morzycka-Wroblewska, E., and Kagnoff, M. F. (1993) Differential cytokine expression by human intestinal epithelial cell lines: regulated expression of interleukin-8. Gastroenterology. 105:1689-1697.
[3] Stallmach, A., v Lampe, B., Matthes, H., Bornhoft, B., and Riecken, E. O. (1992) Diminished expression of integrin adhesion molecules on human colonic epithelial cells during the benign to malign tumor. Gut 33:342-346.

Biological
Cell System Endocrine Cell System
Cell System Gastrointestinal Cell System
Cell Type Epithelial Cells
Species Human (Normal)