Human Peripheral Blood CD19+ B Cells (Positive Selection)

Description

Product Description

B cells, also known as B lymphocytes, are a subtype of lymphocyte in white blood cells ^[1]. They play a critical role in the humoral immunity component of the adaptive immune system by secreting antibodies^[1]. They also function in immune system as antigen presentation cells and by secretion of cytokines^[1].

CD19 (Cluster of Differentiation 19) is an important surface marker for B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. CD19 primarily acts as a B cell co-receptor in conjunction with CD21 ^[2] and CD81. Upon activation, the cytoplasmic tail of CD19 becomes phosphorylated, which leads to binding by Src-family kinases and recruitment of PI-3 kinase.

iXCells Biotechnologies offers CD19+ B Cells isolated from normal human peripheral blood mononuclear cells (PBMCs) using positive immunomagnetic selection. > 90% of the cells are CD19+ as showed by flow cytometric analysis (Figure 1).

Figure 1. Flow cytometric analysis showed that >90% cells are CD19 positive.

Product Details

 

Tissue	Normal human peripheral blood
Package Size	5 x106 cells/vial
Passage Number	P0
Purity	> 90%
Shipped	Cryopreserved
Storage	Liquid nitrogen
Growth Properties	Suspension
Media	Blood Cell Culture Medium (Cat# MD-0007)

 

References

[1] Murphy, Kenneth. 2012. Janeway’s Immunobiology 8^th Edition. New York, NY: Garland Science.

[2] Bradbury LE, Kansas GS, Levy S, Evans RL, and Tedder TF. “The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules”. Journal of Immunology 1992; 149 (9):2841-2850.

 

Download Datasheet