Human Pulmonary Microvascular Endothelial Cells (HPMEC)

Description Product Code Price Quantity Add to Cart
Cryopreserved, 0.5 million cells/vial
10HU-076
$659.00

Product Description

The primary human pulmonary microvascular endothelial cells (HPMEC) form a semiselective barrier that is critical for lung gas exchange and regulation of fluid and solute passage between the blood and interstitial compartments in the lung. They also have metabolic properties that enable it to carry out certain important nonrespiratory function. The HPMEC are among the most important targets of reactive oxygen species elaborated in lung injury [1]. During the course of lung inflammation, neurohumoral mediators and oxidants act on endothelial cells to induce intercellular gaps permissive for transudation of proteinaceous fluid from blood into the interstitium [2]. The increased permeability leads to the hypoxemia associated with adult respiratory distress syndrome and noncardiogenic pulmonary edema [3].

iXCells Biotechnologies provides high quality HPMEC, which are isolated from human lung tissue and cryopreserved at P2, with >0.5 million cells in each vial. HPMEC express vWF/Factor VIII, CD31 (PECAM), and Dil-Ac-LDL by uptake. They are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast, and fungi and can further expand in Endothelial Cell Growth Medium (Cat# MD-0010) under the condition suggested by iXCells Biotechnologies. 

Product Details

  Tissue

  Human lung tissue

  Package Size

  0.5 million cells/vial  

  Passage Number

  P2

  Shipped

  Cryopreserved

  Storage

  Liquid nitrogen

  Growth Properties

  Adherent

  Media

  Endothelial Cell Growth Medium (Cat# MD-0010)

References

[1] Brigham, K. L. (1990) Oxidant stress and adult respiratory distress syndrome. Eur Respir J Suppl 11: 482s-484s.
[2] Moore T. M., Chetham P. M., Kelly J. J., Stevens T. (1998) Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP. Am J Physiol. 275(2 Pt 1):L203-22.
[3] Kelly JJ, Moore TM, Babal P, Diwan AH, Stevens T, Thompson WJ. (1998) Pulmonary microvascular and macrovascular endothelial cells: differential regulation of Ca2+ and permeability. Am J Physiol. 274(5 Pt 1):L810-9.

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[1] Brigham, K. L. (1990) Oxidant stress and adult respiratory distress syndrome. Eur Respir J Suppl 11: 482s-484s.
[2] Moore T. M., Chetham P. M., Kelly J. J., Stevens T. (1998) Signal transduction and regulation of lung endothelial cell permeability. Interaction between calcium and cAMP. Am J Physiol. 275(2 Pt 1):L203-22.
[3] Kelly JJ, Moore TM, Babal P, Diwan AH, Stevens T, Thompson WJ. (1998) Pulmonary microvascular and macrovascular endothelial cells: differential regulation of Ca2+ and permeability. Am J Physiol. 274(5 Pt 1):L810-9.

Biological
Cell System Pulmonary Cell System
Cell System Vascular Cell System
Cell Type Endothelial Cells
Species Human (Normal)